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Image Search Results
Journal: Journal of Experimental & Clinical Cancer Research : CR
Article Title: STAT3/miR-130b-3p/MBNL1 feedback loop regulated by mTORC1 signaling promotes angiogenesis and tumor growth
doi: 10.1186/s13046-022-02513-z
Figure Lengend Snippet: miR-130b-3p promotes angiogenesis via targeting MBNL1. A-D Tsc2 − / − MEFs were infected with lentiviruses harboring control vector (Lv) or MBNL1 overexpression constructs (Lv-MBNL1). A The expression of MBNL1 was determined by western blot. B Cell culture supernatants from the cells were analyzed for VEGF-C by ELISA. (RU, relative unit). C and D The effect on angiogenesis of was detected by tube formation assay ( C , scale bar, 50 μm) and CAM assay ( D ). E–G anti-miR-130b-3p-expressing NTC/T2 null cells were infected with lentivirus harboring shRNAs targeting MBNL1 (shMBNL1-1 and shMBNL1-2) or a control shRNA (shSc). E Cell lysates were subjected to immunoblotting with the indicated antibodies. F and G The angiogenic abilities of the indicated cells were detected by tube formation assay ( F , scale bar, 50 μm) and CAM assay ( G ). Representative images (left panels) and quantifications (right panels). H–K The indicated cells were inoculated subcutaneously into nude mice ( n = 5), and tumor growth was monitored. H Tumor pictures. Scale bar, 1 cm. I Tumor volumes, J Tumor weight. K Tumor tissues were subjected to HE and IHC staining. Scale bar, 50 μm. Data indicate mean ± SD of 3–5 replicates. * P < 0.05; ** P < 0.01; *** P < 0.001; **** P < 0.0001
Article Snippet: The levels of secreted VEGF-C in cell-free supernatant of MBNL1-overexpressing cells and the control cells were quantified using a
Techniques: Infection, Control, Plasmid Preparation, Over Expression, Construct, Expressing, Western Blot, Cell Culture, Enzyme-linked Immunosorbent Assay, Tube Formation Assay, Chick Chorioallantoic Membrane Assay, shRNA, Immunohistochemistry